PAP Associated Domain Containing 7 (PAPD7) (AA 447-462) antibody

Catalog No. ABIN241579

Product Details

Target: PAP Associated Domain Containing 7 (PAPD7)

Binding Specificity: AA 447-462

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: A synthetic peptide containing amino acids 447-462 of human TRF4-1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Panda, Dog, Bat (88%) Hamster, Elephant, Rabbit, Horse, Opossum, Platypus (81%).

Purification: Protein G purified

Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptide containing amino acids 447-462 (GTASLKAVHHMSSPAI) of human TRF4-1, GenBank no NP_008930.1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Panda, Dog, Bat (88%), Hamster, Elephant, Rabbit, Horse, Opossum, Platypus (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: PAP Associated Domain Containing 7 (PAPD7)

Alternative Name: PAPD7 / TRF4-1 (PAPD7 Products)

Synonyms: LAK-1 antibody, LAK1 antibody, POLK antibody, POLS antibody, TRF4 antibody, TRF4-1 antibody, TRF41 antibody, TUTASE5 antibody, TRF4-1b antibody, pols antibody, Pols antibody, DNA polymerase sigma antibody, poly(A) RNA polymerase D7, non-canonical antibody, non-canonical poly(A) RNA polymerase PAPD7 antibody, PAP associated domain containing 7 antibody, Eint_061490 antibody, PAPD7 antibody, LOC100579823 antibody, papd7 antibody, Papd7 antibody

Target Type: Viral Protein

Background: Name/Gene ID: PAPD7

Synonyms: PAPD7, DNA polymerase kappa, DNA polymerase sigma, LAK1, POLS, LAK-1, Terminal uridylyltransferase 5, TRF4, TUTase 5, TRF4-1, TRF41, TUTASE5

Gene ID: 11044

NCBI Accession: NP_008930

UniProt: Q5XG87