tau antibody (pTyr18)

Catalog No. ABIN2443721

There is 1 publication for this product available. The Mouse Monoclonal anti-tau antibody is suitable to detect tau in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for tau antibody (pTyr18) (ABIN2443721)

Target: tau (tau Protein)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This tau antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 9G3

Product Details anti-tau Antibody

Binding Specificity: AA 12-24, pTyr18

Purpose: Phospho-Tau (tyr 18) Mouse Monoclonal Antibody (9G3)

Specificity: The antibody recognizes the human Phospho-Tau protein at the tyrosine 18 phosphorylation site with no detectable cross-reactivity.

Purification: Protein G purified

Immunogen: Peptide

Isotype: IgG2a

Application Details

Application Notes: WB 1:1000-1:5000,IHC 1:1000-1:5000,IF 1:2000-1:5000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-1:5000, IHC 1:1000-1:5000, IF 1:2000-1:5000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

References for anti-tau antibody (ABIN2443721)

Tang, Fesharaki-Zadeh, Takahashi, Nies, Smith, Luo, Chyung, Chiasseu, Strittmatter: "Fyn kinase inhibition reduces protein aggregation, increases synapse density and improves memory in transgenic and traumatic Tauopathy." in: Acta neuropathologica communications, Vol. 8, Issue 1, pp. 96, (2020) (PubMed).

Target Details for tau

Target: tau (tau Protein)

Alternative Name: tau

Background:

Phosphorylated Tau

Tauopathies are a group of diverse dementias and movement disorders which have a common pathological feature in that they all display the presence of intracellular accumulation of abnormal filaments of Tau protein. While the abnormal phosphorylation of Tau on serine and threonine has been well established in the disease process, its phosphorylation on tyrosine 18 has only recently been described as present in Alzheimer's disease (AD) brain.

UniProt: P10636