SMAP1 antibody (AA 132-288)

Catalog No. ABIN2443724

The Rabbit Polyclonal anti-SMAP1 antibody is suitable to detect SMAP1 in samples from Human. It has been validated for WB and IP.

Quick Overview for SMAP1 antibody (AA 132-288) (ABIN2443724)

Target: SMAP1 (Stromal Membrane Associated Protein 1 (SMAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-SMAP1 Antibody

Binding Specificity: AA 132-288

Purpose: SMAP1 Rabbit Polyclonal Antibody (IP,WB)

Specificity: The antibody recognizes the human small ArfGAP1 Isoform 1 protein (aa 132-288).

Application Details

Application Notes: WB 1:5000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: WB 1:5000, IP: native conditions. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for SMAP1

Target: SMAP1 (Stromal Membrane Associated Protein 1 (SMAP1))

Alternative Name: SMAP1

Background:

Small ArfGAP1, stromal membrane associated protein-1,

Stromal membrane associated protein-1 (SMAP1, also known as small ArfGAP1) is a GTPase activating protein that plays a role in clathrin-dependent endocytosis. It may also play a role in erythropoiesis. SMAP1 is known to interact with ARF6 and the clathrin heavy chains via the clathrin box-like motif. SMAP1 is expressed in bone marrow, adrenal gland, trachea, lymph node, spinal cord, peripheral blood leukocytes, thyroid and stomach.

UniProt: Q8IYB5