AHI1 antibody (C-Term)

Catalog No. ABIN2443749

The Mouse Monoclonal anti-AHI1 antibody (Clone J5) (ABIN2443749) specifically detects AHI1 in WB, IHC and IP. The antibody is reactive with Mouse samples.

Quick Overview for AHI1 antibody (C-Term) (ABIN2443749)

Target: AHI1 (Abelson Helper Integration Site 1 (AHI1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This AHI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: J5

Product Details anti-AHI1 Antibody

Binding Specificity: C-Term

Purpose: Ahi-1 Mouse Monoclonal Antibody (J5)

Specificity: A specific and sensitive antibody against Ahi-1. The antibody recognizes the C-terminal of the murine Ahi-1. There is no cross-reactivity with human Ahi-1

Purification: Protein G purified

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100μL: WB 1-2 μg/mL, IP 1-2 μg of antibody to 200-600 μg of proteins, IHC 1-5 μg/mL. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for AHI1

Target: AHI1 (Abelson Helper Integration Site 1 (AHI1))

Alternative Name: Ahi-1

Background:

anti-ahi-1, anti-ahi 1, anti-ahi1, Jouberin , Abelson helper integration site 1 protein , AHI-1

Abelson helper integration site 1 (Ahi-1) is highly expressed in the most primitive normal hematopoietic cells, in brain neurons and in kidney. Ahi-1 is a unique protein with a SH3 domain, multiple SH3 binding sites and a WD40-repeat domain, which are all known to be important mediators of protein-protein interactions. Defects in Ahi-1 are the cause of Joubert syndrome type 3 (JBTS3).

UniProt: Q8K3E5