Chromogranin A antibody (N-Term)

Catalog No. ABIN2443753

This Rabbit Polyclonal antibody specifically detects Chromogranin A in WB. It exhibits reactivity toward Human and Mammalian.

Quick Overview for Chromogranin A antibody (N-Term) (ABIN2443753)

Target: Chromogranin A (CHGA)

Reactivity: Human, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Chromogranin A Antibody

Binding Specificity: AA 4-16, N-Term

Purpose: Chromogranin-A (N-term) Rabbit Polyclonal Antibody

Specificity: The antibody recognizes an epitope in N-terminal (AA 4-16) of Chromogranine-A

Immunogen: N-terminal peptide (AA 4-16)

Application Details

Application Notes: WB 1:2000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:2000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Chromogranin-A

Background:

hCGA, parathyroid secretory protein 1, CHGA

Chromogranin-A is a secreted protein known as a ""prohormone"" which is found in endocrine cells and neurons and may be released upon stimulation. It is thought to be involved in the formation of dense core granules, where it may be processed to form the bioactive peptide serpinin, which is thought to regulate the biogenesis of dense core granules in endocrine cells. Chromogranin-A is used as a biomarker in patients with neuroendocrine tumors. Recent publications show that incomplete processing of Chromogranin-A may be linked to diabetic retinopathy

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling