DYKDDDDK Tag antibody (C-Term, N-Term)

Catalog No. ABIN2443789

This Mouse Monoclonal antibody specifically detects DYKDDDDK Tag in WB, ELISA, IP, IHC and DB.

Quick Overview for DYKDDDDK Tag antibody (C-Term, N-Term) (ABIN2443789)

Target: DYKDDDDK Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Dot Blot (DB)

Clone: FG4R

Product Details anti-DYKDDDDK Tag Antibody

Binding Specificity: C-Term, N-Term

Purpose: FLAG Tag Mouse Monoclonal Antibody (FG4R)

Sequence: DYKDDDDK

Specificity: The antibody recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.

Purification: Protein G purified

Immunogen: The antibody was raised against DYKDDDDK (FLAG) synthetic peptide conjugated to KLH.

Isotype: IgG2b

Application Details

Application Notes: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: FLAG Tag

Target Type: Tag

Background: Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size the protein's biochemical properties seem not to be affected by the tag protein. FLAG-tag, or FLAG octapeptide, a polypeptide consisting of eight amino acids (DYKDDDDK) can be added to a protein using recombinant DNA technology, therefore, the tag can be detected with the fused protein in many different assays that require recognition by an antibody.