Nerve Growth Factor antibody

Catalog No. ABIN2443811

The Rat Monoclonal anti-Nerve Growth Factor antibody is suitable to detect Nerve Growth Factor in samples from Mouse. It has been validated for WB, ELISA and IF.

Quick Overview for Nerve Growth Factor antibody (ABIN2443811)

Target: Nerve Growth Factor (NGF)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: NGF30

Product Details anti-Nerve Growth Factor Antibody

Purpose: Nerve growth Factor (NGF) Rat Monoclonal Antibody (NGF30)

Specificity: This antibody recognizes mouse NGF monomer protein (MW 13 kDa) by WB. It does not cross react with EGF, BSA, APO or insulin.

Purification: Protein G purified

Immunogen: polymerized NGF

Isotype: IgG2a

Application Details

Application Notes: WB 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:200. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Background:

Beta-nerve growth factor, Beta-NGF, NGF

Nerve growth factor (NGF) is a small secreted protein which induces the differentiation and survival of particular target neurons. It is perhaps the prototypical growth factor, in that it is one of the first to be described. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. There is some data that shows that NGF can be transported from the axon tip to soma. One of the most important molecules in the nervous system, nerve growth factor and its other neurotrophin family members control the development of the nervous system in the embryo and the maintenance of nervous tissue and neural transmission in the adult.

UniProt: P01139

Pathways: Regulation of Cell Size