Aconitase 1 antibody (full length)

Catalog No. ABIN2443828

This Rabbit Polyclonal antibody specifically detects Aconitase 1 in WB. It exhibits reactivity toward Human and Mouse.It has been mentioned in 1 publication

Quick Overview for Aconitase 1 antibody (full length) (ABIN2443828)

Target: Aconitase 1 (ACO1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aconitase 1 Antibody

Binding Specificity: full length

Purpose: Iron Regulatory Protein 1 (IRP1) Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the gull length IRP1 protein

Immunogen: Full length protein

Application Details

Application Notes: WB 1:2000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: WB 1:2000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

References for anti-Aconitase 1 antibody (ABIN2443828)

Deng, Manz, Torti, Torti: "Iron-responsive element-binding protein 2 plays an essential role in regulating prostate cancer cell growth." in: Oncotarget, Vol. 8, Issue 47, pp. 82231-82243, (2017) (PubMed).

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: Iron Regulatory Protein 1 (IRP1)

Background:

Iron-responsive element-binding proteins, IRE-BP, IRBP, IRP, IFR

The iron regulatory proteins IRP1 and IRP2 are involved in posttranscriptional regulation of cellular iron metabolism by binding to mRNA iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. IRP-1 is a bifunctional soluble protein that functions as an IRE-binding protein or as the cytoplasmic isoform of aconitase.

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis