CYTH2
Reactivity: Human, Rat
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 680
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
If reconstituted with deionized water in 100 μL: WB: 1:2000, IHC: 1:100-1:250. Optimal dilution has to be determined by the user.
Buffer
Lyophilized serum
Preservative
Without preservative
Storage
4 °C,-20 °C,-80 °C
Storage Comment
Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
Expiry Date
24 months
Target
Cytohesin 2 (CYTH2)
Alternative Name
ARNO (Cytohesin-2)
Background
ARNO / Cytohesin-2 acts as guanine-nucleotide exchange factor (GEF) for ADP-ribosylation factor such as ARF1, ARF3 and ARF6. Both isoforms (1 and 2) of ARNO / Cytohesin-2 are located in the cytoplasm and in cell membrane after being recruited through their association with ARL4A, ARL4C and ARL4D