RAB27A antibody

Catalog No. ABIN2443836

The Rabbit Polyclonal anti-RAB27A antibody (ABIN2443836) specifically detects RAB27A in WB and IP. The antibody is reactive with Human samples.

Quick Overview for RAB27A antibody (ABIN2443836)

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB27A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-RAB27A Antibody

Purpose: Rab27a Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the human Rab27a protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB: 1:1000 (Recommended blocking solution: Donkey Serum 20 % ). IP: native conditions with 0.2 % TritonX-100. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for RAB27A

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Alternative Name: Rab27a

Background:

Ras-related protein Rab-27A, RAB27A, GS2, HsT18676, RAB27, RAM, member RAS oncogene family

Ras-related protein Rab-27a (Rab27a) belongs to the small GTPase superfamily. The protein is membrane-bound and is involved in protein transport and small GTPase-mediated signal transduction. Rab27a plays a role in cytotoxic granule exocytosis in lymphocytes. Rab27a is widely expressed with considerable expression in pancreatic islets and pituitary tissue, and low expression in the brain. Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2), a rare autosomal recessive disorder.

UniProt: P51159

Pathways: Peptide Hormone Metabolism