beta Actin antibody

Catalog No. ABIN2443850

The Mouse Monoclonal anti-beta Actin antibody is suitable to detect beta Actin in samples from Human, Mouse, Rat, Chicken and Rabbit. It has been validated for WB, ELISA, IHC and DB.

Quick Overview for beta Actin antibody (ABIN2443850)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Clone: BA3R

Product Details anti-beta Actin Antibody

Purpose: Beta-Actin Mouse Monoclonal Antibody (BA3R)

Specificity: The antibody recognizes native and denatured forms of β-Actin (~42 kDa)

Purification: Protein G purified

Isotype: IgG2b

Application Details

Application Notes: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Beta-Actin

Background: β-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. The β-actin gene is often stably and constitutively expressed at high levels in most tissues and cells. Proteins such as β-actin, involved in maintenance of basic cellular function, are often referred to as housekeeping proteins. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction