Enkephalin antibody (N-Term)

Catalog No. ABIN2443860

The Rabbit Polyclonal anti-Enkephalin antibody (ABIN2443860) specifically detects Enkephalin in WB. The antibody is reactive with Mammalian and Cow samples.

Quick Overview for Enkephalin antibody (N-Term) (ABIN2443860)

Target: Enkephalin (PENK) (Proenkephalin (PENK))

Reactivity: Mammalian, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Enkephalin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Enkephalin Antibody

Binding Specificity: AA 63-70, N-Term

Purpose: Proenkephalin-A (N-term) Rabbit Polyclonal Antibody

Specificity: The antibody recognizes an epitope in N-terminal (AA 63-70) of Proenkephalin A

Immunogen: N-terminal peptide (AA 63-70)

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for Enkephalin

Target: Enkephalin (PENK) (Proenkephalin (PENK))

Alternative Name: Proenkephalin-A

Background:

Proenkephalin (PENK), PEA

Vertebrate endogenous opioid neuropeptides are released by post-translational proteolytic cleavage of precursor proteins. Proenkephalin-A is one of the three opioid neuropeptide precursor molecules of the opioid pentapeptides Met- and Leu-enkephalin, which compete with and mimic the effects of opiate drugs. Upon cleavage, each proenkephalin peptide results in the generation of four copies of Met-enkephalin, two extended copies of Met-enkephalin, and one copy of Leu-enkephalin. Proenkephalin opioid neuropeptide is one of the key neurobiological substrates of addiction disorders implicated in reward, mood regulation, stress response, and motor function.

UniProt: P01211

Pathways: Stem Cell Maintenance