ROBO4 antibody

Catalog No. ABIN2443871

The Guinea Pig Polyclonal anti-ROBO4 antibody is suitable to detect ROBO4 in samples from Human and Mouse. It has been validated for WB and FACS.

Quick Overview for ROBO4 antibody (ABIN2443871)

Target: ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))

Reactivity: Human, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This ROBO4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ROBO4 Antibody

Purpose: Robo4 Guinea pig Polyclonal Antibody

Specificity: The antibody recognizes Robo-4 protein.

Immunogen: Recombinant protein

Application Details

Application Notes: WB 1:1000,FACS 1:300

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000, FACS 1:300. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for ROBO4

Target: ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))

Alternative Name: Robo4

Background:

Robo, roundabout guidance receptor 4, mRobo4-4CI5

The Robo (Roundabout) family of receptors is known for its guidance role during development. Studies have shown that one of the family members, Robo4, is specifically expressed in the vascular endothelium and may provide directional cues to migrating endothelial cells. Recent evidence shows that Robo4-deficient mice have increased angiogenesis, suggesting that Robo4 may play a role in restricting vascular sprouting.

UniProt: Q6EIG7