YY1 antibody

Catalog No. ABIN2443872

Cited in 1 publication. The Chicken Polyclonal anti-YY1 antibody (ABIN2443872) specifically detects YY1 in WB and IF. The antibody is reactive with Human, Mouse, Rat, Sheep and Pig samples.

Quick Overview for YY1 antibody (ABIN2443872)

Target: YY1 (YY1 Transcription Factor (YY1))

Reactivity: Human, Mouse, Rat, Sheep, Pig

Host: Chicken

Clonality: Polyclonal

Conjugate: This YY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-YY1 Antibody

Purpose: YY1 Chicken Polyclonal Antibody

Specificity: The antibody recognizes the Transcription Factor YY1, in WB, the antibody recognizes the O-glycosylated, the phosphorylated, and the post-translationally unmodified protein in cellular and tissue lysates.

Purification: Purified

Immunogen: Recombinant human YY1

Application Details

Application Notes: WB 1:3000,IF 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 30 μL: WB 1:3000 - 1:8000, IF 1:500 - 1:1000 (in tissue paraffin sections). Optimal dilution has to be determined by the user.

Buffer: Lyophilized purified total IgY in PBS pH 7.4

Preservative: Sodium azide, Streptomycin

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

References for anti-YY1 antibody (ABIN2443872)

Gelev, Zabolotny, Lange, Hiromura, Yoo, Orlando, Kushnir, Horikoshi, Paquet, Bachvarov, Schaffer, Usheva: "A new paradigm for transcription factor TFIIB functionality." in: Scientific reports, Vol. 4, pp. 3664, (2015) (PubMed).

Target Details for YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Alternative Name: YY1

Background: YY1 is a ubiquitous and multifunctional zinc-finger transcription factor involved in the activation or repression of a large number of genes that are critical for basic biological processes of cell growth, development, differentiation, cell cycle and even programmed cell death (apoptosis). YY1 is an essential protein, deletion of YY1 leads to lethality in mouse embryos and disruption of one allele causing developmental defects.

UniProt: P25490