VEGF antibody

Catalog No. ABIN2443873

This Chicken Polyclonal antibody specifically detects VEGF in WB and IF. It exhibits reactivity toward Human, Mouse and Pig.

Quick Overview for VEGF antibody (ABIN2443873)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivity: Human, Mouse, Pig

Host: Chicken

Clonality: Polyclonal

Conjugate: This VEGF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-VEGF Antibody

Purpose: VEGF Chicken Polyclonal Antibody

Specificity: The antibody recognizes the partially reduced homodimmers and multimers that VEGF spontaneously forms. In reduced samples, the antibody recognizes the reduced monomer.

Purification: Purified

Immunogen: Recombinant human VEGF

Application Details

Application Notes: WB 1:1000,IF 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 30 μL: WB 1:1000 - 1:8000, IF 1:500 - 1:2000 (in cells fixed with paraformaldehyde). Optimal dilution has to be determined by the user.

Buffer: Lyophilized purified total IgY in PBS pH 7.4

Preservative: Sodium azide, Streptomycin

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF

Background: Vascular endothelial growth factor (VEGF) is a dimeric glycoprotein that stimulates vasculogenesis and angiogenesis. VEGF belongs to a family of closely related growth factors that share a conserved pattern of eight cysteine residues and bind to common VEGF receptors. The VEGF family members bind in an overlapping manner to three receptor tyrosine kinases, VEGFR1, VEGFR2 and VEGFR3. Specifically, VEGF mediates angiogenic response by the activation of VEGFR2. VEGF is produced at high levels in endothelial cells but its expression can be found in other cell types, including fibroblasts, inflammatory cells, and many tumor cells, often in response to increasing tumor hypoxia via the HIF-1a pathway. Overexpression of VEGF can contribute to diseases such as cancer and vascular disease in the retina, also, VEGF is a cause of susceptibility to microvascular complications of diabetes type 1 such as diabetic retinopathy, diabetic nephropathy and diabetic neuropathy.

UniProt: P15692