SAMD10 antibody

Catalog No. ABIN2443897

This Rabbit Polyclonal antibody specifically detects SAMD10 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for SAMD10 antibody (ABIN2443897)

Target: SAMD10 (Sterile alpha Motif Domain Containing 10 (SAMD10))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAMD10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SAMD10 Antibody

Purpose: SamD10 Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the protein SamD10. The antibody can also recognize SamD12.

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for SAMD10

Target: SAMD10 (Sterile alpha Motif Domain Containing 10 (SAMD10))

Alternative Name: SamD10

Background:

Sterile alpha motif (SAM) domain-containing protein 10, Hyphen D10, HYPD10, Aveugle

SamD10 and SamD12 are two recently discovered homolog proteins consisting of a SAM domain. These proteins are also known as Hyphen (HYP) or Aveugle (Ave) and they have been demonstrated to interact with the SAM domain of the Connector enhancer of KSR (CNK) downstream of RAS but a a step upstream of RAF. A role for these proteins downstream of EGFR during eye and wing development of Drosophila has also been demonstrated. Heterodimerization of CNK/SAM has been shown to facilitate the recruitment of a KSR/MEK complex to CNK and bridge KSR/MEK to RAF which greatly enhances RAS-mediated RAF activation.

UniProt: Q9BYL1