SAMD12 antibody

Catalog No. ABIN2443898

The Rabbit Polyclonal anti-SAMD12 antibody is suitable to detect SAMD12 in samples from Human and Mouse. It has been validated for WB.

Quick Overview for SAMD12 antibody (ABIN2443898)

Target: SAMD12 (Sterile alpha Motif Domain Containing 12 (SAMD12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAMD12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SAMD12 Antibody

Purpose: SamD12 Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the protein SamD12. The antibody does not cross-react with SamD10.

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for SAMD12

Target: SAMD12 (Sterile alpha Motif Domain Containing 12 (SAMD12))

Alternative Name: SamD12

Background:

Sterile alpha motif (SAM) domain-containing protein 12, Hyphen D12, HYPD12, Aveugle

SamD10 and SamD12 are two recently discovered homolog proteins consisting of a SAM domain. These proteins are also known as Hyphen (HYP) or Aveugle (Ave) and they have been demonstrated to interact with the SAM domain of the Connector enhancer of KSR (CNK) downstream of RAS but a step upstream of RAF. A role for these proteins downstream of EGFR during eye and wing development of Drosophila has also been demonstrated. Heterodimerization of CNK/SAM has been shown to facilitate the recruitment of a KSR/MEK complex to CNK and bridge KSR/MEK to RAF which greatly enhances RAS-mediated RAF activation.

UniProt: Q8N8I0