GAPDH antibody

Catalog No. ABIN2443912

This Mouse Monoclonal antibody specifically detects GAPDH in WB, ELISA, IHC and DB. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Rabbit, Hamster, Saccharomyces cerevisiae, E. coli and Sf9 insect cell line.

Quick Overview for GAPDH antibody (ABIN2443912)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Hamster, Saccharomyces cerevisiae, E. coli, Sf9 insect cell line

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Clone: GA1R

Product Details anti-GAPDH Antibody

Purpose: GAPDH Mouse Monoclonal Antibody (GA1R)

Specificity: The antibody recognizes native and denatured forms of GAPDH.

Characteristics: Specific detection of E coli BL21

Purification: Protein G purified

Isotype: IgG1

Application Details

Application Notes: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-2,000, IHC 1:500-2000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Glyceraldehyde 3-phosphate dehydrogenase

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a 37KDa glycolytic enzyme constitutively and stably expressed in almost all tissues. This enzyme catalyzes the sixth step of glycolysis by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Proteins such as GAPDH involved in maintenance of basic cellular function are often referred to as housekeeping proteins. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.

UniProt: P04406