Clathrin, Light Chain (N-Term) antibody

Catalog No. ABIN2443922

The Rabbit Polyclonal anti-Clathrin, Light Chain antibody is suitable to detect Clathrin, Light Chain in samples from Human, Mouse and Rat. It has been validated for WB and IF.

Quick Overview for Clathrin, Light Chain (N-Term) antibody (ABIN2443922)

Target: Clathrin, Light Chain

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details

Binding Specificity: N-Term

Purpose: Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the N-terminal portion of Clathrin Light Chain

Purification: Immunogen affinity purified

Immunogen: peptide in N term

Application Details

Application Notes: IF 1:100,WB 1:1000-1:2000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 200 μL: IF 1:100 to 1:500, WB 1:1000-1:2000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized immunogen affinity purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details

Target: Clathrin, Light Chain

Alternative Name: Clathrin Light Chain

Background:

Clathrin light chain A, Lca

Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.

UniProt: P09496