VAT1 antibody (N-Term)

Catalog No. ABIN2443931

The Rabbit Polyclonal anti-VAT1 antibody is suitable to detect VAT1 in samples from Human. It has been validated for WB.

Quick Overview for VAT1 antibody (N-Term) (ABIN2443931)

Target: VAT1 (Vesicle Amine Transport 1 (VAT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VAT1 Antibody

Binding Specificity: AA 1-16, N-Term

Purpose: VAT1 (N-terminal) Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the N-terminal of the human Rab27a protein (aa1-16).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB: 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for VAT1

Target: VAT1 (Vesicle Amine Transport 1 (VAT1))

Alternative Name: VAT1

Background:

VAT1, VATI, vesicle amine transport 2

Vesicle Amine Transport protein-1 (VAT1) plays a role in calcium-regulated keratinocyte activation in epidermal repair mechanisms through its ATPase activity. It is expressed in the brain, glioblastoma cells and expression has been detected following wounding. The expression of VAT1 is calcium ion-dependent.

UniProt: Q99536