GFP Tag antibody (N-Term)

Catalog No. ABIN2443932

The Mouse Monoclonal anti-GFP Tag antibody (Clone GF28R) (ABIN2443932) specifically detects GFP Tag in WB, ELISA, IHC, IP and DB. The antibody is reactive with Aequorea victoria samples.

Quick Overview for GFP Tag antibody (N-Term) (ABIN2443932)

Target: GFP Tag

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB)

Clone: GF28R

Product Details anti-GFP Tag Antibody

Binding Specificity: N-Term

Purpose: GFP Tag Mouse Monoclonal Antibody (GF28R)

Specificity: The antibody recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP.

Purification: Protein G purified

Immunogen: GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugates.

Isotype: IgG2b

Application Details

Application Notes: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for GFP Tag

Target: GFP Tag

Target Type: Tag

Background: Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. The green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is involved in the bioluminescence of the jellyfish. When expressed in prokaryotic cells, GFP produces a fluorescent product, making GFP useful in monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.

UniProt: P42212