Leishmania A2 protein (A2) antibody

Catalog No. ABIN2443977

The Mouse Monoclonal anti-Leishmania A2 protein antibody (Clone C9) (ABIN2443977) specifically detects Leishmania A2 protein in IF, IP and WB. The antibody is reactive with Leishmania donovani (L. donovani) and Leishmania samples.

Quick Overview for Leishmania A2 protein (A2) antibody (ABIN2443977)

Target: Leishmania A2 protein (A2)

Reactivity: Leishmania donovani (L. donovani), Leishmania

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Clone: C9

Product Details

Purpose: Leishmania A2 Mouse Monoclonal Antibody (C9)

Sequence: QSVSVGPGPL

Specificity: A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence can also be used as tag if cloned with the protein of interest. This sequence is particular to the parasite and therefore the antibody has no cross-reactivity with mammalian proteins. There is no cross-reactivity with other Leishmania species.

Purification: Protein G purified

Immunogen: The anti-A2 antibody recognizes only the amastigote form of the A2 protein. The anti-A2 antibody recognizes the sequence QSVGPLSVGP from the parasite, Leishmania donovani.

Isotype: IgG1

Application Details

Application Notes: WB 1:100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:100. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details

Target: Leishmania A2 protein (A2)

Alternative Name: Leishmania A2

Background: Leishmania donovani is a protozoa parasite responsible for human visceral leishmaniasis. During their life cycle the parasites exist in 2 forms: as flagellated promastigote within the sandfly vector and as non-flagellated amastigote in the macrophage phagolysosomal compartment of the mammalian host. A2 protein is an amastigote-specific virulence factor that is required for Leishmania donovani parasite survival in a mammalian host.

UniProt: A4HZU7