Tubulin antibody (cleaved, EEVGVD Motif)

Catalog No. ABIN2444034

The Rabbit Polyclonal anti-Tubulin antibody (ABIN2444034) specifically detects Tubulin in WB and IHC. The antibody is reactive with Human, Mouse and Cow samples.

Quick Overview for Tubulin antibody (cleaved, EEVGVD Motif) (ABIN2444034)

Target: Tubulin (TUB)

Reactivity: Human, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Tubulin Antibody

Binding Specificity: cleaved, EEVGVD Motif

Purpose: Alpha-Tubulin cleaved by caspase-6 Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the sequence (EEVGVD) in tubulin protein. It is specific for Caspase 6 cleaved α-Tubulin. The antibody was tested in IHC and western blots of samples with Alzheimer disease (AD) (Bovine brain sample, human samples, cells lines, mice hippocampus and whole brains). When tested in null mice with IHC and WB, no immunoreactivity was observed.

Immunogen: EEVGVD peptide

Application Details

Application Notes: WB 1:1000,IHC 1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: WB 1:1000, IHC 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for Tubulin

Target: Tubulin (TUB)

Alternative Name: Tubulin

Background:

α-Tubulin cleaved by casp 6

Tubulin is the major constituent of microtubules. Microtubules are assembled from dimers of α- and β-tubulin. α-Tubulin cleaved by caspase-6 can be found in neurons, neurofibrillary tangles, neutrophil threads, and neuritic plaques in Alzheimer disease. α-Tubulin has been found to associate with the axonal microtubule-associated protein Tau, indicating that the cleavage of the functional α-Tubulin C-terminal domain by caspase-6 could alter the state of microtubules in neurons.

UniProt: Q71U36