OPTN antibody (AA 115-130)

Catalog No. ABIN2451621

This Rabbit Polyclonal antibody specifically detects OPTN in WB, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for OPTN antibody (AA 115-130) (ABIN2451621)

Target: OPTN (Optineurin (OPTN))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPTN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-OPTN Antibody

Binding Specificity: AA 115-130

Specificity: Human optineurin amino acids 115-130 (KGKSERSSEDPTDDSR)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey (100%) Gibbon (94%).

Purification: Immunoaffinity purified

Immunogen: Human optineurin amino acids 115-130 (KGKSERSSEDPTDDSR). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey (100%), Gibbon (94%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS,  pH 7.4, 50 % glycerol, 0.5 mg/mL BSA, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for OPTN

Target: OPTN (Optineurin (OPTN))

Alternative Name: OPTN / Optineurin

Background: Name/Gene ID: OPTN

Synonyms: OPTN, ALS12, E3-14.7K-interacting protein, FIP-2, HIP-7, HYPL, Huntingtin yeast partner L, HIP7, TFIIIA-INTP, NEMO-related protein, NRP, Optineurin, FIP2, GLC1E

Gene ID: 10133

UniProt: Q96CV9

Pathways: M Phase