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HHV6 gQ1 (AA 3-422) antibody

Reactivity: Human Herpesvirus 6 (HHV-6) IC, FACS, IF, IP, ELISA, WB Host: Mouse Monoclonal 119 unconjugated
Catalog No. ABIN2452012
  • Target
    HHV6 gQ1
    Binding Specificity
    AA 3-422
    Reactivity
    Human Herpesvirus 6 (HHV-6)
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Immunochromatography (IC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
    Specificity
    Reacts with gQ1 of HHV6A and HHV6B. However, this antibody is not recommended for IP of HHV-6B due to conformation specificity.
    Purification
    Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions.
    Purity
    90-95 % pure by SDS-PAGE.
    Sterility
    Sterile filtered
    Immunogen
    His6-tagged recombinant gQ1 of HHV-6A encoding 3-422 amino acids expressed in E. coli.
    Clone
    119
    Isotype
    IgG
  • Application Notes
    1) Western blotting: 1/500~1/1,000 dilution
    2) Immunoprecipitation (assay dependent)
    3) Imunofluorescence staining and Immunocytochemistry: 1/100~1/3,200 dilution
    4) Flow Cytometry: 1/100
    5) ELISA (assay dependent)
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS (x1), 50 % glycerol. Azide and carrier free.
    Preservative
    Azide free
    Storage
    -20 °C
    Storage Comment
    Upon arrival centrifuge briefly and store at -20 C.
  • Tang, Hayashi, Maeki, Yamanishi, Mori: "Human herpesvirus 6 glycoprotein complex formation is required for folding and trafficking of the gH/gL/gQ1/gQ2 complex and its cellular receptor binding." in: Journal of virology, Vol. 85, Issue 21, pp. 11121-30, (2011) (PubMed).

    Tang, Kawabata, Yoshida, Oyaizu, Maeki, Yamanishi, Mori: "Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth." in: Virology, Vol. 407, Issue 2, pp. 360-7, (2010) (PubMed).

    Huang, Li, Sadaoka, Tang, Yamamoto, Yamanishi, Mori: "Human herpesvirus 6 envelope cholesterol is required for virus entry." in: The Journal of general virology, Vol. 87, Issue Pt 2, pp. 277-85, (2006) (PubMed).

    Mori, Akkapaiboon, Yonemoto, Koike, Takemoto, Sadaoka, Sasamoto, Konishi, Uchiyama, Yamanishi: "Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46." in: Journal of virology, Vol. 78, Issue 9, pp. 4609-16, (2004) (PubMed).

    Akkapaiboon, Mori, Sadaoka, Yonemoto, Yamanishi: "Intracellular processing of human herpesvirus 6 glycoproteins Q1 and Q2 into tetrameric complexes expressed on the viral envelope." in: Journal of virology, Vol. 78, Issue 15, pp. 7969-83, (2004) (PubMed).

    Mori, Akkapaiboon, Yang, Yamanishi: "The human herpesvirus 6 U100 gene product is the third component of the gH-gL glycoprotein complex on the viral envelope." in: Journal of virology, Vol. 77, Issue 4, pp. 2452-8, (2003) (PubMed).

    Mori, Yang, Akkapaiboon, Okuno, Yamanishi: "Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex associates with human CD46." in: Journal of virology, Vol. 77, Issue 8, pp. 4992-9, (2003) (PubMed).

  • Target
    HHV6 gQ1
    Target Type
    Viral Protein
    Background
    Background: Human herpesvirus 6 (HHV-6) is the common collective name for Human herpesvirus 6A (HHV-6A) and Human herpesvirus 6B (HHV-6B). These closely related viruses are two of the nine herpesviruses known to have humans as their primary host. HHV-6A and HHV-6B are double stranded DNA viruses within the betaherpesvirinae subfamily and of the genus Roseolovirus. HHV-6A and HHV-6B infects almost all of the human populations tested. The overall nucleotide sequence identity between HHV- 6A and HHV-6B is 90 % and they are now classified as distinct species. HHV-6A has been described as more neurovirulent, and as such is more frequently found in patients with neuroinflammatory diseases such as multiple sclerosis. HHV-6B primary infection is the cause of the common childhood illness exanthem subitum (also known as roseola infantum or sixth disease). Additionally, HHV-6B reactivation is common in transplant recipients, which can cause several clinical manifestations such as encephalitis, bone marrow suppression and pneumonitis. gQ1 encoded by the U100 gene of HHV6 is glycoprotein, complexes with gH, gL and gQ2 to form HHV6A ligand to CD46 receptor and HHV6B ligand to CD134 receptor. It is expressed in two different forms: an 80- kDa form (gQ1-80K) and a 74- kDa form (gQ1-74K) - only gQ1-80K, but not gQ1-74K, forms the ligand complex with gQ2, gH, and gL. It associates with lipid rafts.
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