HIV-1 p24 antibody (Biotin)

Catalog No. ABIN2452028

Product Details anti-HIV-1 p24 Antibody (Biotin)

Target: HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIV-1 p24 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Characteristics: This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.

Purification: Purified

Sterility: Sterile filtered

Isotype: IgG

Application Details

Application Notes: 1. Western blotting
2. Immunoprecipitaiton
3. Immunofluorescence staining
4. ELISA
Not tested in other application

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-HIV-1 p24 antibody (Biotin) (ABIN2452028)

Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).

Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).

Tanaka, Saitoh, Nakata, Shinagawa: "A simple method for overproduction and purification of p24 Gag protein of human immunodeficiency virus type 1." in: Microbiology and immunology, Vol. 36, Issue 8, pp. 823-31, (1993) (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

Target Details for HIV-1 p24

Target: HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

Alternative Name: HIV-1 p24 (HIV-1 p24 Products)

Target Type: Viral Protein

Background: HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction. p24 is used as a marker antigen for observing the patient's condition after treatment, as it indicates the amount of virus in the blood. The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B, and was highly purified by several steps of chromatography. Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells.