CBX1 antibody (AA 176-185)

Catalog No. ABIN2452034

The Rabbit Polyclonal anti-CBX1 antibody has been validated for WB, IF and ChIP-seq. It is suitable to detect CBX1 in samples from Human and Hamster. There are 3+ publications available.

Quick Overview for CBX1 antibody (AA 176-185) (ABIN2452034)

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Reactivity: Human, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-CBX1 Antibody

Binding Specificity: AA 176-185

Cross-Reactivity (Details): Expected to react with mouse, chicken, zebra fish, Xenopus, Drosophila orthologs due to the sequence identity of the immunogen.

Characteristics: The product is prepared by immunizing rabbit with the synthetic peptide CNEDDDKKDDKN including the C-terminal sequence (176-185) of human HP1beta and purified by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi.

Purification: Affinity purified

Sterility: Sterile filtered

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: 1/2,000~1/10,000
2) Immunofluorescence staining
3) Chromatin immunoprecipitation (ChIP)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.44 mg/mL

Buffer: 0.12 M sodium phosphate buffer ( pH 7.4), 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References for anti-CBX1 antibody (ABIN2452034)

Lomberk, Wallrath, Urrutia: "The Heterochromatin Protein 1 family." in: Genome biology, Vol. 7, Issue 7, pp. 228, (2007) (PubMed).

Wang, Koyama, Nishida, Haraguchi, Reith, Tsukamoto: "The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4028-40, (2006) (PubMed).

Kametaka, Takagi, Hayakawa, Haraguchi, Hiraoka, Yoneda: "Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 12, pp. 1231-42, (2002) (PubMed).

Target Details for CBX1

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Alternative Name: HP1 beta/cbx1

Background: Heterochromatin protein 1 (HP1) is a major component of heterochromatin which plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1beta is encoded by the Chromobox homolog 1 (CBX1) gene located on chromosome 17q21.32. HP1beta has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions.

UniProt: P83916