IZUMO1 antibody

Catalog No. ABIN2452040

The Rat Monoclonal anti-IZUMO1 antibody has been validated for WB, IF and IP. It is suitable to detect IZUMO1 in samples from Human and Mouse. There are 5+ publications available.

Quick Overview for IZUMO1 antibody (ABIN2452040)

Target: IZUMO1 (Izumo Sperm-Egg Fusion 1 (IZUMO1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IZUMO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: #125

Product Details anti-IZUMO1 Antibody

Cross-Reactivity (Details): Not tested in other species.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Mouse sperm

Isotype: IgG2a kappa

Application Details

Application Notes: 1. Western blotting: 1/1,000 dilution
2. Immunoprecipitation: 1/100 dilution
3. Immunofluorescence staining: 1/250 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol. Azide- and carrier protein-free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly, aliquote and store at -20 C.

References for anti-IZUMO1 antibody (ABIN2452040)

Barbaux, Ialy-Radio, Chalbi, Dybal, Homps-Legrand, Do Cruzeiro, Vaiman, Wolf, Ziyyat: "Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion." in: Scientific reports, Vol. 10, Issue 1, pp. 5335, (2020) (PubMed).

Inoue, Hamada, Kamikubo, Hirata, Kataoka, Yamamoto, Ikawa, Okabe, Hagihara: "Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion." in: Development (Cambridge, England), Vol. 140, Issue 15, pp. 3221-9, (2013) (PubMed).

Satouh, Inoue, Ikawa, Okabe: "Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1." in: Journal of cell science, Vol. 125, Issue Pt 21, pp. 4985-90, (2013) (PubMed).

Ikawa, Tokuhiro, Yamaguchi, Benham, Tamura, Wada, Satouh, Inoue, Okabe: "Calsperin is a testis-specific chaperone required for sperm fertility." in: The Journal of biological chemistry, Vol. 286, Issue 7, pp. 5639-46, (2011) (PubMed).

Inoue, Kasahara, Ikawa, Okabe: "Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse." in: PLoS ONE, Vol. 5, Issue 4, pp. e10301, (2010) (PubMed).

Target Details for IZUMO1

Target: IZUMO1 (Izumo Sperm-Egg Fusion 1 (IZUMO1))

Alternative Name: IZUMO1

Background: Key words: Acrosome reaction, Membrane fusion, Protein trafficking, IZUMO1, Sperm-egg fusion

Molecular Weight: 44,885 Da

UniProt: Q9D9J7, Q8IYV9