Nas6/p28 antibody

Catalog No. ABIN2452055

There are 3+ publications for this product available. The Rabbit Polyclonal anti-Nas6/p28 antibody is suitable to detect Nas6/p28 in samples from Saccharomyces cerevisiae. It has been validated for IP and WB.

Quick Overview for Nas6/p28 antibody (ABIN2452055)

Target: Nas6/p28

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Not tested in other species.

Purification: Affinity purified

Immunogen: Recombinant yeast Nas6 expressed in E. coli

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: 5000 fold dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 1 mg/mL BSA, 0.09 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Saeki, Toh-E, Kudo, Kawamura, Tanaka: "Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle." in: Cell, Vol. 137, Issue 5, pp. 900-13, (2009) (PubMed).

Hori, Kato, Saeki, DeMartino, Slaughter, Takeuchi, Toh-e, Tanaka: "cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome." in: Gene, Vol. 216, Issue 1, pp. 113-22, (1998) (PubMed).

Hershko, Ciechanover: "The ubiquitin system." in: Annual review of biochemistry, Vol. 67, pp. 425-79, (1998) (PubMed).

Target Details

Target: Nas6/p28

Background: Background: The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation. The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. NAS6 (p28) is a proteasome-interacting protein which acts as a chaperon to assemble the base sub-complex of the 19S RP. NAS6 is composed of 226 amino acid residues with calculated molecular masses of 24.4kD.