Nestin antibody

Catalog No. ABIN2452056

This anti-Nestin antibody is a Rat Monoclonal antibody detecting Nestin in IHC and ICC. Suitable for Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Nestin antibody (ABIN2452056)

Target: Nestin (NES)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 7A3

Product Details anti-Nestin Antibody

Specificity: Specific to mouse Nestin.

Cross-Reactivity (Details): Not tested in other species.

Characteristics: The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Mouse E16 embryonic cerebral cortex extracts

Isotype: IgG2b kappa

Application Details

Application Notes: 1. Immunocytochemistry
2. Immunohistochemistry
This antibody doesn't work in immunoblotting.
Other applications are not tested.

This antibody is very useful for immunostaining of mouse embryonic brain because it is rat antibody. Rat antibody has very low background in immunostaining using mouse tissues and is also useful for double-staining with mouse and rabbit antibodies.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-Nestin antibody (ABIN2452056)

Gilyarov: "Nestin in central nervous system cells." in: Neuroscience and behavioral physiology, Vol. 38, Issue 2, pp. 165-9, (2008) (PubMed).

Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Nestin is an intermediate filament protein that is expressed in stem cells and progenitor cells in the mammalian central nervous system (CNS) during development. Nestin is replaced in the adult organism by other intermediate filament proteins, however, it may be re-expressed under certain pathological conditions such as ischemia, inflammation, brain injury, and neoplastic transformation. Nestin has been detected in many kinds of tumors, especially in tumors derived from the CNS, therefore it is considered to be a marker for cancer stem cells in neurogenic tumors.

UniProt: Q6P5H2