PSMD12 antibody

Catalog No. ABIN2452114

This Rabbit Polyclonal antibody specifically detects PSMD12 in WB and IP. It exhibits reactivity toward Saccharomyces cerevisiae and has been mentioned in 3+ publications.

Quick Overview for PSMD12 antibody (ABIN2452114)

Target: PSMD12 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 12 (PSMD12))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD12 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PSMD12 Antibody

Characteristics: Product: Rabbit polyclonal antibody affinity purified with recombinant Rpn5p

Purification: Affinity purified

Immunogen: Recombinant yeast Rpn5p expressed in E. coli

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: ~1000 fold dilution
2) Immunoprecipitation Not tested for other applications

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCl, 10 mM Tris-HCl pH 7.4, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: To avoid repeated freezing and thawing, store the antibody solution in aliquots.

Storage: -20 °C

References for anti-PSMD12 antibody (ABIN2452114)

Isono, Nishihara, Saeki, Yashiroda, Kamata, Ge, Ueda, Kikuchi, Tanaka, Nakano, Toh-e: "The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome." in: Molecular biology of the cell, Vol. 18, Issue 2, pp. 569-80, (2007) (PubMed).

Finley, Tanaka, Mann, Feldmann, Hochstrasser, Vierstra, Johnston, Hampton, Haber, Mccusker, Silver, Frontali, Thorsness, Varshavsky, Byers, Madura, Reed, Wolf, Jentsch, Sommer, Baumeister, Goldberg et al.: "Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. ..." in: Trends in biochemical sciences, Vol. 23, Issue 7, pp. 244-5, (1998) (PubMed).

Hershko, Ciechanover: "The ubiquitin system." in: Annual review of biochemistry, Vol. 67, pp. 425-79, (1998) (PubMed).

Target Details for PSMD12

Target: PSMD12 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 12 (PSMD12))

Alternative Name: Rpn5

Background: Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation. Rpn5p is essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7. It consists of 445 amino acids with molecular weight of 51,768.

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway