Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
Specificity
ADROPIN antibody is human, mouse and rat reactive. Despite its predicted molecular weight, ADROPIN often migrates at a higher than expected molecular weight in SDS-PAGE.
Purification
ADROPIN antibody is affinity chromatography purified via peptide column.
Immunogen
ADROPIN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ADROPIN.
ENHO
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
CC1133F5
unconjugated
Application Notes
ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Can be stored at 4 °C for three months and -20 °C, stable for up to one year.
ADROPIN is a recently identified protein that has been implicated in the maintenance of energy homeostasis and insulin resistance (1-3). ADROPIN expression is regulated by energy status and dietary nutrient content and is altered by obesity and regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma (PPAR-gamma) (1). ADROPIN levels increase with dietary fat content (2). ADROPIN has also been proposed to play a role in the regulation of endothelial function (3).