DPF3 antibody

Catalog No. ABIN2458080

The Rabbit Polyclonal anti-DPF3 antibody (ABIN2458080) specifically detects DPF3 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DPF3 antibody (ABIN2458080)

Target: DPF3 (D4, Zinc and Double PHD Fingers, Family 3 (DPF3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DPF3 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPF3.

Application Details

Application Notes: DPF3 antibody can be used for detection of DPF3 by ELISA at 1:12500. DPF3 antibody can be used for detection of DPF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DPF3 antibody at -20 °C.

Target Details for DPF3

Target: DPF3 (D4, Zinc and Double PHD Fingers, Family 3 (DPF3))

Alternative Name: DPF3

Background: DPF3 is a muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). DPF3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.

Molecular Weight: 40 kDa

Gene ID: 8110

NCBI Accession: NP_036206

UniProt: Q92784