PIGK antibody

Catalog No. ABIN2458515

Product Details anti-PIGK Antibody

Target: PIGK (GPI8) (GPI-anchor transamidase (GPI8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIGK.

Application Details

Application Notes: PIGK antibody can be used for detection of PIGK by ELISA at 1:62500. PIGK antibody can be used for detection of PIGK by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PIGK antibody at -20 °C.

Target Details for PIGK

Target: PIGK (GPI8) (GPI-anchor transamidase (GPI8))

Alternative Name: PIGK (GPI8 Products)

Synonyms: GPI8 antibody, 3000001O05Rik antibody, zgc:86702 antibody, phosphatidylinositol glycan anchor biosynthesis class K S homeolog antibody, phosphatidylinositol glycan anchor biosynthesis class K antibody, phosphatidylinositol glycan anchor biosynthesis, class K antibody, pigk.S antibody, PIGK antibody, Pigk antibody, pigk antibody

Background: PIGK is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. PIGK is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 43 kDa

Gene ID: 10026

NCBI Accession: NP_005473

UniProt: Q92643