CKM antibody

Catalog No. ABIN2458586

The Rabbit Polyclonal anti-CKM antibody has been validated for WB and ELISA. It is suitable to detect CKM in samples from Human, Mouse, Rat and Dog.

Quick Overview for CKM antibody (ABIN2458586)

Target: CKM (Creatine Kinase, Muscle (CKM))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CKM Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CKM.

Application Details

Application Notes: CKM antibody can be used for detection of CKM by ELISA at 1:1562500. CKM antibody can be used for detection of CKM by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CKM antibody at -20 °C.

Target Details for CKM

Target: CKM (Creatine Kinase, Muscle (CKM))

Alternative Name: CKM

Background: CKM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CKM is a member of the ATP: guanido phosphotransferase protein family.The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 43 kDa

Gene ID: 1158

NCBI Accession: NP_001815

UniProt: P06732