HPRT1 antibody

Catalog No. ABIN2459200

This Rabbit Polyclonal antibody specifically detects HPRT1 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for HPRT1 antibody (ABIN2459200)

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPRT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HPRT1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HPRT1.

Application Details

Application Notes: HPRT1 antibody can be used for detection of HPRT1 by ELISA at 1:12500. HPRT1 antibody can be used for detection of HPRT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store HPRT1 antibody at -20 °C.

Target Details for HPRT1

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HPRT1

Background: HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate.HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).

Molecular Weight: 24 kDa

Gene ID: 3251

NCBI Accession: NP_000185

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process