Aminomethyltransferase antibody

Catalog No. ABIN2459732

The Rabbit Polyclonal anti-Aminomethyltransferase antibody is suitable to detect Aminomethyltransferase in samples from Human, Mouse, Rat and Dog. It has been validated for WB and ELISA.

Quick Overview for Aminomethyltransferase antibody (ABIN2459732)

Target: Aminomethyltransferase (AMT)

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aminomethyltransferase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Aminomethyltransferase Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AMT.

Application Details

Application Notes: AMT antibody can be used for detection of AMT by ELISA at 1:12500. AMT antibody can be used for detection of AMT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store AMT antibody at -20 °C.

Target Details for Aminomethyltransferase

Target: Aminomethyltransferase (AMT)

Alternative Name: AMT

Background: The enzyme system for cleavage of glycine (glycine cleavage system, EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). Glycine encephalopathy (GCE) may be due to a defect in any one of these enzymes.The enzyme system for cleavage of glycine (glycine cleavage system, EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase, MIM 238300), H protein (a lipoic acid-containing protein, MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase, MIM 238331). Glycine encephalopathy (GCE, MIM 605899) may be due to a defect in any one of these enzymes.

Molecular Weight: 44 kDa

Gene ID: 275

NCBI Accession: NP_000472

UniProt: P48728