BCL2L1 antibody

Catalog No. ABIN2460189

The Rabbit Polyclonal anti-BCL2L1 antibody (ABIN2460189) specifically detects BCL2L1 in WB and ELISA. The antibody is reactive with Human and Dog samples.

Quick Overview for BCL2L1 antibody (ABIN2460189)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BCL2L1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BCL2L1.

Application Details

Application Notes: BCL2L1 antibody can be used for detection of BCL2L1 by ELISA at 1:312500. BCL2L1 antibody can be used for detection of BCL2L1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store BCL2L1 antibody at -20 °C.

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: BCL2L1

Background: BCL2L1 encodes a protein which belongs to the BCL-2 protein family. The proteins encoded by BCL2L1 are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis.The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

Molecular Weight: 26 kDa, 19 kDa

Gene ID: 598

NCBI Accession: NP_612815

UniProt: Q07817

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling