MAP3K8 antibody

Catalog No. ABIN2460190

This Rabbit Polyclonal antibody specifically detects MAP3K8 in WB and ELISA. It exhibits reactivity toward Human, Rat and Dog.

Quick Overview for MAP3K8 antibody (ABIN2460190)

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Reactivity: Human, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MAP3K8 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAP3K8.

Application Details

Application Notes: MAP3K8 antibody can be used for detection of MAP3K8 by ELISA at 1:1562500. MAP3K8 antibody can be used for detection of MAP3K8 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MAP3K8 antibody at -20 °C.

Target Details for MAP3K8

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Alternative Name: MAP3K8

Background: MAP3K8 is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.

Molecular Weight: 53 kDa

Gene ID: 1326

NCBI Accession: NP_005195

UniProt: P41279

Pathways: PI3K-Akt Signaling, TCR Signaling