TRIM32 antibody

Catalog No. ABIN2460275

The Rabbit Polyclonal anti-TRIM32 antibody has been validated for WB and ELISA. It is suitable to detect TRIM32 in samples from Human, Mouse, Rat and Dog.

Quick Overview for TRIM32 antibody (ABIN2460275)

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TRIM32 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRIM32.

Application Details

Application Notes: TRIM32 antibody can be used for detection of TRIM32 by ELISA at 1:62500. TRIM32 antibody can be used for detection of TRIM32 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TRIM32 antibody at -20 °C.

Target Details for TRIM32

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Alternative Name: TRIM32

Background: TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Molecular Weight: 72 kDa

Gene ID: 22954

NCBI Accession: NP_036342

UniProt: Q13049

Pathways: Negative Regulation of intrinsic apoptotic Signaling