PAWR antibody

Catalog No. ABIN2460368

The Rabbit Polyclonal anti-PAWR antibody is suitable to detect PAWR in samples from Human, Mouse, Rat and Dog. It has been validated for WB, IHC and ELISA.

Quick Overview for PAWR antibody (ABIN2460368)

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAWR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PAWR Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAWR.

Application Details

Application Notes: PAWR antibody can be used for detection of PAWR by ELISA at 1:62500. PAWR antibody can be used for detection of PAWR by western blot at 0.2-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PAWR antibody at -20 °C.

Target Details for PAWR

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Alternative Name: PAWR

Background: The tumor suppressor WT1 represses and activates transcription. Anti-Prostate Apoptosis Response Protein Par-4 (PAWR) is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.

Molecular Weight: 37 kDa

Gene ID: 5074

NCBI Accession: NP_002574

UniProt: O75796