NFIB antibody

Catalog No. ABIN2460545

The Rabbit Polyclonal anti-NFIB antibody has been validated for WB and ELISA. It is suitable to detect NFIB in samples from Human, Mouse, Rat and Dog.

Quick Overview for NFIB antibody (ABIN2460545)

Target: NFIB (Nuclear Factor I/B (NFIB))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NFIB Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIB.

Application Details

Application Notes: NFIB antibody can be used for detection of NFIB by ELISA at 1:312500. NFIB antibody can be used for detection of NFIB by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFIB antibody at -20 °C.

Target Details for NFIB

Target: NFIB (Nuclear Factor I/B (NFIB))

Alternative Name: NFIB

Background: NFIB is a member of the nuclear factor I family of nuclear proteins, which are known to be involved in viral and cellular transcription. NFIB includes the proposed DNA binding and dimerization domain. NFIB recognizes and binds the palindromic sequence 5'-

Molecular Weight: 47 kDa

Gene ID: 4781

NCBI Accession: NP_005587

UniProt: O00712