ALF antibody

Catalog No. ABIN2460589

This Rabbit Polyclonal antibody specifically detects ALF in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ALF antibody (ABIN2460589)

Target: ALF

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALF.

Application Details

Application Notes: ALF antibody can be used for detection of ALF by ELISA at 1:312500. ALF antibody can be used for detection of ALF by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ALF antibody at -20 °C.

Target Details

Target: ALF

Background: The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.

Molecular Weight: 50 kDa, 52 kDa, 132 kDa

Gene ID: 11036

NCBI Accession: NP_751946

UniProt: Q9UNN4