ALF (N-Term) antibody

Catalog No. ABIN2777620

This Rabbit Polyclonal antibody specifically detects ALF in WB. It exhibits reactivity toward Human, Cow, Dog, Guinea Pig, Horse, Rabbit and Rat.

Quick Overview for ALF (N-Term) antibody (ABIN2777620)

Target: ALF

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Sequence: VIPAGRTLPS FTTAELGTSN SSANFTFPGY PIHVPAGVTL QTVSGHLYKV

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 83%, Horse: 100%, Human: 100%, Rabbit: 91%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ALF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ALF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 453 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: ALF

Background: The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.
Alias Symbols: ALF
Protein Interaction Partner: GAB1, NKX2-3, ZBTB3, TLX3, MAFF, RBFOX2, HMG20A, PAX9, SIX6, MAFG, HOXC11, HOXC9, HOXA5, TLX2, EMX2, EMX1, CEBPE, ATF4, ASCL3, GTF2A2, SUB1, ID3, CSNK2A2, CSNK2A1, TBP,
Protein Size: 453

Molecular Weight: 50 kDa

Gene ID: 11036

NCBI Accession: NM_172196, NP_751946

UniProt: Q9UNN4