PHF1 antibody

Catalog No. ABIN2460639

The Rabbit Polyclonal anti-PHF1 antibody (ABIN2460639) specifically detects PHF1 in WB, ELISA and IHC. The antibody is reactive with Human, Rat and Dog samples.

Quick Overview for PHF1 antibody (ABIN2460639)

Target: PHF1 (PHD Finger Protein 1 (PHF1))

Reactivity: Human, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PHF1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHF1.

Application Details

Application Notes: PHF1 antibody can be used for detection of PHF1 by ELISA at 1:1562500. PHF1 antibody can be used for detection of PHF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PHF1 antibody at -20 °C.

Target Details for PHF1

Target: PHF1 (PHD Finger Protein 1 (PHF1))

Alternative Name: PHF1

Background: PHF1 has significant sequence similarity with Drosophila Polycomblike. It contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. This gene encodes a protein with significant sequence similarity to Drosophila Polycomblike. The encoded protein contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. Two transcript variants have been found for this gene.

Molecular Weight: 62 kDa

Gene ID: 5252

NCBI Accession: NP_077084

UniProt: O43189