PHF1 antibody (N-Term)

Catalog No. ABIN6242526

The Rabbit Polyclonal anti-PHF1 antibody is suitable to detect PHF1 in samples from Mouse. It has been validated for WB.

Quick Overview for PHF1 antibody (N-Term) (ABIN6242526)

Target: PHF1 (PHD Finger Protein 1 (PHF1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHF1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB48341

Product Details anti-PHF1 Antibody

Binding Specificity: AA 164-197, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PHF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 164-197 amino acids from the N-terminal region of human PHF1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for PHF1

Target: PHF1 (PHD Finger Protein 1 (PHF1))

Alternative Name: PHF1

Background: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980). Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.

Molecular Weight: 62106

UniProt: O43189