POLE3 antibody

Catalog No. ABIN2460641

The Rabbit Polyclonal anti-POLE3 antibody has been validated for WB and ELISA. It is suitable to detect POLE3 in samples from Human, Mouse, Rat and Dog.

Quick Overview for POLE3 antibody (ABIN2460641)

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLE3 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLE3.

Application Details

Application Notes: POLE3 antibody can be used for detection of POLE3 by ELISA at 1:312500. POLE3 antibody can be used for detection of POLE3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLE3 antibody at -20 °C.

Target Details for POLE3

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Alternative Name: POLE3

Background: POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.

Molecular Weight: 17 kDa

Gene ID: 54107

NCBI Accession: NP_059139

UniProt: Q9NRF9

Pathways: DNA Damage Repair