POU4F3 antibody

Catalog No. ABIN2460646

This Rabbit Polyclonal antibody specifically detects POU4F3 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog and Zebrafish (Danio rerio).

Quick Overview for POU4F3 antibody (ABIN2460646)

Target: POU4F3 (POU Domain, Class 4, Transcription Factor 3 (POU4F3))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU4F3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-POU4F3 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU4F3.

Application Details

Application Notes: POU4F3 antibody can be used for detection of POU4F3 by ELISA at 1:312500. POU4F3 antibody can be used for detection of POU4F3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POU4F3 antibody at -20 °C.

Target Details for POU4F3

Target: POU4F3 (POU Domain, Class 4, Transcription Factor 3 (POU4F3))

Alternative Name: POU4F3

Background: POU4F3 is capable of activating both BDNF and NT-3 promoters in inner ear sensory epithelial cell lines. Mutant POU4F3 loses most of its transcriptional activity and most of its ability to bind to DNA. The mutation causes autosomal-dominant nonsyndromic hearing loss and eventually leads to hair cell morbidity in affected family members.

Molecular Weight: 37 kDa

Gene ID: 5459

NCBI Accession: NP_002691

UniProt: Q15319

Pathways: Sensory Perception of Sound