ATP7A antibody

Catalog No. ABIN2460808

This Rabbit Polyclonal antibody specifically detects ATP7A in WB and ELISA. It exhibits reactivity toward Human and Dog.

Quick Overview for ATP7A antibody (ABIN2460808)

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP7A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ATP7A Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP7A.

Application Details

Application Notes: ATP7A antibody can be used for detection of ATP7A by ELISA at 1:1562500. ATP7A antibody can be used for detection of ATP7A by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ATP7A antibody at -20 °C.

Target Details for ATP7A

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Alternative Name: ATP7A

Background: The ATP7A gene encodes the Menkes copper-translocating P-type ATPase, a ubiquitous protein that regulates the absorption of copper in the gastrointestinal tract. Inside cells, this protein has a dual function: it delivers copper to cuproenzymes in the Golgi compartment and effluxes excess copper. The trafficking mechanism and catalytic activity combine to facilitate absorption and intercellular transport of copper. Menkes disease, a systemic copper deficiency disorder, is caused by mutations in the ATP7A gene.

Molecular Weight: 30 kDa, 163 kDa, 70 kDa

Gene ID: 538

UniProt: Q762B6

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process