FHL1 antibody

Catalog No. ABIN2460931

This Rabbit Polyclonal antibody specifically detects FHL1 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for FHL1 antibody (ABIN2460931)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-FHL1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FHL1.

Application Details

Application Notes: FHL1 antibody can be used for detection of FHL1 by ELISA at 1:312500. FHL1 antibody can be used for detection of FHL1 by western blot at 0.1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store FHL1 antibody at -20 °C.

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: FHL1

Background: LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may play an important role during the early stages of skeletal muscle differentiation, specifically in alpha5beta1-integrin-mediated signaling pathways.

Molecular Weight: 32 kDa

Gene ID: 2273

NCBI Accession: NP_001440

UniProt: Q6IB30